[Wolf Run Wildlife Refuge | wildlife sanctuaries Kentucky | Nicholasville, KY, USA Wolf Run Wildlife Refuge is a 501(c)3, non profit charity, specializing in the rescue of wildlife, promoting education, and offering lifelong sanctuary to animals that cannot be released into the wild. Wolf Run is the eldest sanctuary in Kentucky of it's kind with 40 years of lifesaving experience. Located in Jessamine County, WR currently houses 32 wolves, coyotes, bobcats, servals, deer, fox, parrots and many others. ]